Two-dimensional, rotational-echo double-resonance NMR of cell culture metabolism.
نویسندگان
چکیده
منابع مشابه
Introduction to rotational-echo, double-resonance NMR
( ) ABSTRACT: Rotational-echo, double-resonance nuclear magnetic resonance REDOR is a magic-angle spinning experiment for measuring internuclear distances between heteronuclear spin pairs. The basic principles of the REDOR technique are presented. The experimental requirements necessary for quantitative REDOR measurements are also provided. Q 1998 John Wiley & Sons, Inc. Concepts Magn Reson 10:...
متن کاملMeasurement of N–H bond lengths by rotational-echo double-resonance NMR spectroscopy
A method based on rotational-echo double-resonance (REDOR) NMR is proposed to measure H–N bond lengths under magic-angle-spinning. Protons are selectively polarized and evolve under the frequency-switched Lee– Goldburg (FSLG) homonuclear decoupling, while two N p pulses are applied to restore H–N dipolar interactions. Such a method relies on the H–N dipolar couplings and requires no knowledge o...
متن کاملX–H rotational-echo double-resonance NMR for torsion angle determination of peptides
C–H and N–H rotational-echo double-resonance (REDOR) NMR is developed for determining torsion angles in peptides. The distance between an X spin such as C or N and a proton is measured by evolving the proton magnetization under REDOR-recoupled X–H dipolar interaction. The proton of interest is selected through its directly bonded heteronuclear spin Y. The sidechain torsion angle v1 is extracted...
متن کاملCompensation for pulse imperfections in rotational-echo double-resonance NMR by composite pulses and EXORCYCLE.
We describe a simple method to compensate for pulse-angle errors in rotational-echo double resonance (REDOR) experiments for determining heteronuclear distances in solids. By using composite 180 degrees pulses on the unobserved dephasing spin and EXORCYCLE for the single pi pulse on the observed channel, the REDOR curve becomes much less sensitive to pulse-angle errors. Both improvements are de...
متن کاملComparison of Three-Dimensional Double-Echo Steady-State Sequence with Routine Two-Dimensional Sequence in the Depiction of Knee Cartilage
Introduction: There are some routine two-dimensional sequences, including short tau inversion recovery (STIR), T2-weighted fast-spin echo (T2W-FSE), and proton-density fast spin-echo for diagnosing osteoarthritis and lesions of the knee cartilage. However, these sequences have some disadvantages, such as long scan time, inadequate spatial resolution, and suboptimal tis...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1993
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)36849-8